Methane hydrate can be used as a carrier to store and transport natural gas. However, they generate slowly under natural conditions,so it is necessary to improve the rate of methane hydrate formation.To this end, the effect of the amphoteric surfactant LAD⁃40 on the methane hydrate formation rate in various mass fractions (10~500 μg/g) was examined. The results show that when the pressure is constant, compared with the nucleation temperature of methane hydrate formation under pure water conditions, adding any mass fractions of LAD⁃40, the nucleation temperature of methane hydrate changes little, which fails to improve the thermodynamic conditions of methane hydrate formation; because of the existence of LAD⁃40, the gas⁃liquid contact area was increased, the nucleation time was shortened, and the hydrate form in the reactor phase. As the LAD⁃40 mass fraction increases, the methane hydrate formation rate increases linearly, and the gas consumption does not change much, however, compared with methane hydrate formation under pure water conditions, it is significantly improved.